Seahorse XF Glycolytic Rate Assay Kit. The XF Glycolytic Rate Assay provides a precise measure of glycolysis in live cells, revealing transient responses and rapid metabolic switches not discernible in endpoint lactate assays. Run on a Seahorse XF Analyzer, this assay quantifies proton efflux specific to glycolysis.
The Glycolytic Rate Assay kit contains the materials sufficient for six full-plate assays and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF/XFe96 and XFe24 Analyzers and the XFp kit is for use with the Agilent Seahorse XF.
103344-100 Seahorse XF Glycolytic Rate Assay Kit EA 6 pouches1 XFe96, XF96, XFe24 S7805A Seahorse XF Glycolytic Rate Assay Starter Pack EA 1 XF Glycolytic Rate Assay kit 1 30-mL 1M HEPES buffer 1 500-mL XF base medium, phenol red free XFe96, XF96, XFe24 103591-100 Seahorse XFp Real-Time ATP Rate Assay Kit EA 6 pouches2 XFp, Seahorse XF Glycolytic Rate Assay Starter Pack. This product contains one each of the following products: XF Glycolytic Rate Assay kit (103344-100), XF DMEM medium pH 7.4, 500mL (103575-100), XF 1.0 M Glucose Solution 50 mL (103577-100), XF 100 mM Pyruvate Solution 50 mL (103578-100), and XF 200 mM Glutamine Solution 50 mL (103579-100).
The Seahorse XFp Glycolytic Rate Assay Kit includes six foil pouches, each containing Rot/AA mix and 2-DG. The kit reagents are sufficient for six complete XFp Glycolytic Rate Assays in an Agilent Seahorse XFp Cell Culture Miniplate. Kit Shipping and Storage The product ships at ambient temperature, and should be stored at room temperature.
Glycolytic rate assay kit, Seahorse Glycolytic Rate Assay …
Glycolytic rate assay kit, Seahorse Glycolytic Rate Assay …
Agilent Seahorse XF Assay Kits and Reagents, Glycolytic rate assay kit, Seahorse Glycolytic Rate Assay …
Once the injection ports are loaded, gently place cartridge in a CO 2-free incubator at 37°C until ready to begin the assay . XF Glycolysis Stress Test Kit User Manual (XF24) 14 Running the assay with the XF Glycolysis Stress Test Software App 1. Open the XF software 2. In the Seahorse Apps drop-down menu, choose XF Glycolysis Kit 3.
The Seahorse XF Glycolysis Stress Test Kit measures the capacity of the glycolytic pathway by driving cells toward glycolysis and assesses the ability of study models to increase glycolytic activity to meet metabolic and bioenergetic demands. Refer to the Seahorse XF Glycolytic Rate Assay for a measure of lactate-associated proton efflux.
The XF Glycolytic Rate Assay provides a precise measure of glycolysis in live cells, revealing transient responses and rapid metabolic switches not discernible in endpoint lactate assays . Run on a Seahorse XF Analyzer, this assay quantifies proton efflux specific to glycolysis . This assay should be performed using XF pH-defined media.
Seahorse XF Real-Time ATP Rate Assay Kit. The Agilent Seahorse XF ATP Real-Time rate assay is the only assay that measures and quantifies the rate of ATP (adenosine triphosphate) production from glycolysis and mitochondria simultaneously using label-free technology in live cells, in real time. Real-time kinetic quantification delivers a dynamic …
Data analysis tool, the Seahorse XF Glycolytic Rate Assay Report Generator, is provided to simplify and transform raw data into organized, shareable summary reports anyone can understand. The assay is for use with an XFe96, XF96, or XFe24 Analyzer. not compatible with XF24 and XF24-3 analyzers. For more information, please see the Agilent Seahorse XF …
